rabbit anti phospho akt1  (Bioss)

Journal: Scientific Reports

Article Title: BPIFB4 rs4339026 A > G polymorphism impacts COPD susceptibility in the Kashi population, China

doi: 10.1038/s41598-025-98599-4

Figure Lengend Snippet: Expression of BPIFB4 and key proteins in the PI3K/AKT pathway in COPD mice. ( a ) Illustration for COPD mouse models. ( b , c ) Western blot analysis of BPIFB4 ( n = 10/group), PI3K, p-PI3K, AKT1, and p-AKT1 ( n = 3/group) in the lung tissues of COPD mice and controls. ( d ) BPIFB4 mRNA expression assessed by RT-qPCR ( n = 10/group). ( e , f ) Statistical significance: ns represented not significant, *** P < 0.001, and **** P < 0.0001, versus controls.

Article Snippet: Membranes were blocked with 5% non-fat milk or BSA for 1 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies targeting BPIFB4 (ab168171, Abcam, UK), PI3K (20584-1-AP, Proteintech, China), p-PI3K (20584-1-AP, Proteintech, China), AKT1 (2938, CST, USA), and p-AKT1 (4060, CST, USA).

Techniques: Expressing, Western Blot, Quantitative RT-PCR